Hydrodynamic Characterization by Analytical Ultracentrifugation and Dynamic Light Scattering
نویسندگان
چکیده
A laboratory-made sample of the polysaccharide xylinan (acetan) has been further characterized with respect to ( i ) purity, (ii) molar mass and polydispersity, and (iii) gross conformation by a combination of hydrodynamic measurements (sedimentation velocity and equilibrium analytical ultracentrifugation, viscometry, and dynamic light scattering) in aqueous NaCl (I = 0.10 mol. L-'). Sedimentation velocity diagrams recorded using Schlieren optics revealed highlypure material sedimenting as a single boundary [ s ; ~ , ~ = 9.5 +0.7) S; k, = (273 k 112) rnL/g]. The hypersharp nature of these boundaries is symptomatic ofa polydisperse and highly nonideal (in the thermodynamic sense) system. Low speed sedimentation equilibrium in the analytical ultracentrlfuge using Rayleigh interference optics and two diflerent types of extrapo-
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